the protein phosphatase 2a in binding peptides and polynukleotide chemistry

摘要

Peptide, smaller than 30, preferably 20, amino acids (aa), that binds specifically in vitro to type 2A protein phosphatase holoenzyme (PPA2), or one of its subunits, is new. Independent claims are also included for the following: (1) polypeptide (Ia) comprising repetitions of (I); (2) polynucleotides (II) that encode (I), and multimers of (II); (3) cellular expression vector that contains (II) and regulatory sequences; and (4) purified, mono- or poly-clonal antibodies (Ab) that bind specifically to (I). ACTIVITY : Cytostatic; Virucide; Antiparasitic. No details of tests for these activities are given. MECHANISM OF ACTION : (I) induce apoptosis, especially of tumor cells, in a p53-independent manner. They inhibit competitively interaction of the native protein, from which they are derived, with PPA2 or its subunits, resulting in deregulation of phosphorylation (and thus activation) of Bcl-2. Binding of (I) also reduces cellular transcription of Jun B in virus-infected cells.