Analysis and classification ,in particular of biological or biochemical objects,on the basis of time-lapse images,applicable in cytometric time-lapse cell

摘要

Among the proposals provided is a method for the analysis and classification of objects of interest, for example biological or biochemical objects, on the basis of time-lapse images, for example for use in time-lapse analysis in image-base cytometry. Images of the objects of interest, for example cells, are recorded at different moments in time and these images are subjected to a segmentation process to identify image elements as object representations or sub-object representations of objects or sub-objects of interest of objects of interest. Identified object representations or sub-object representations are then associated with one another in images of the time series and are identified as representations of the same object or sub-object or as the result of an object or sub-object. First features manifesting themselves in individual images are detected and second features manifesting themselves in a plurality of images recorded at different times are detected. The individual objects or sub-objects identified in the digital images of the series are classified on the basis of at least one classifier relating to at least one second feature, and this classification process is used as the basis for or as part of a further analysis process in relation to at least one query of interest.