Method of changing a globular protein structure into a fibrillar protein structure.

摘要

The method comprising the steps of providing a globular protein, forming a solution containing the globular protein, adding a detergent to the solution containing the globular protein, and applying the solution to a molecular sizing column with a pore size of at least 70 kDa. The column is preferably selected from a SuperdexRTM200 or a HW555, and the detergent is preferably selected from SDS or Zwittergent 3-14. The globular protein is preferably selected from albumin, fibronectin or recombinant capsid proteins VP1, VP2, or VP3 of the foot and mouth disease virus, or precursor or chimeric proteins thereof. The proteins may have a use in medicine, particularly in the treatment of cancer, or they may be used as vaccine adjuvants.