OVEREXPRESSION, PURIFICATION, AND CHARACTERIZATION OF THERMOLABILE PHOSPHATASE

摘要

PPROBLEM TO BE SOLVED: To provide compositions for an alkaline phosphatase and methods for overexpression and purification of thermolabile Antarctic phosphatase (TAP). PSOLUTION: The compositions for an alkaline phosphatase and the methods for overexpression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides, and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65C and efficiency of dephosphorylation activity at approximately neutral pH. PCOPYRIGHT: (C)2011,JPO&INPIT