Overexpression, purification and characterization of thermolabile phosphatases

摘要

Constitution and thermal unstable antakuteitsukuhosuhuataze for alkaline phosphatase (TAP) method of excess revelation and refinement is offered. Nuclear acid and the sugar, you can list to the use of TAP, deviation from phosphorylation of peptide and the protein. As for TAP which is stated in this bill, it possesses many advantages than thermal instability and at approximately neutral pH the phosphatase from other resource in the 65C in regard to the performance of deviation from phosphorylation activity.