Fluorescent annular peptide, the production method and use of these peptides in enzyme active measuring of the protease enzyme

摘要

As for this invention, arrangement below: s1 x1 s2 x2(In formula, S₁ is first target peptide arrangement of protease enzyme E₁; S₂ is second target peptide arrangement of protease enzyme E₂; As for S₁ and S₂ to be possible identical being also to differ; Particular first peptide arrangement, S₁ and particular second peptide arrangement S₂ becomes, including 4 or 14 amino acids; As for E₁ and E₂ to be possible in the same protease enzyme, or to the protease enzyme which differs to be suitable; X_{1 the} fluorescent donor basis is the probe which supports; And X₂ fluorescence or the non fluorescent receptor basis is the probe which supports)Including, it regards the annular peptide which becomes. As for these peptides it can use for the active measurement of the protease enzyme.