TRAP VECTOR AND METHOD FOR GENE TRAPPING USING THE SAME

摘要

PPROBLEM TO BE SOLVED: To provide a random mutation ES cloning technology by a gene trapping method. PSOLUTION: The trap vector includes a loxP sequence, composed, in order, of a reverse repetitive sequence 1, a spacer sequence and a reverse repetitive sequence 2, and the variant loxP sequence which has a mutation introduced into the sequence of a part of the reverse repetitive sequence 1 or the sequence of a part of the reverse repetitive sequence 2 of the loxP sequence. The gene trapping method comprises the introduction of the trap vector into embryonic stem cells. The embryonic stem cells and non-human transgenic animals which contain the vector are obtained. PCOPYRIGHT: (C)2011,JPO&INPIT