Protein complex detection methods, and protein complex detection kit

摘要

Provide can also be detected complex formation of proteins that bind to the transcription factor, or transcription factors and, with other proteins, protein complexes and detection kit protein complex detection method. In a protein synthesis system capable of producing a protein by gene expression, the chimeric protein linked DBD X protein (12) and (11) and (10), functional peptide that converts the transcriptional repressor any transcription factor (22) I produce chimeric protein linked Y protein (21) and with (20a). Y protein X and protein (11) (21), to form a complex, the complex (40) is formed as a result. (40), by transcriptional repression action with (22) functional peptide, suppress in trans the transcription of the reporter gene (31) this complex. By checking this transcriptional repression, to detect the protein complex formed by (21) Y protein X and protein (11).