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METHOD FOR DETECTION AND QUANTIFICATION OF PROTEIN, AND REAGENT KIT FOR DETECTION AND QUANTIFICATION OF PROTEIN

机译:蛋白质检测和定量的方法以及蛋白质检测和定量的试剂盒

摘要

PROBLEM TO BE SOLVED: To provide an easy, rapid and sensitive method for detection and quantification of a protein with high reproducibility with superior operability, easily performed even by an examiner inexperienced in biochemical or chemical experiments, and detecting the presence of a trace protein and quantifying the protein by a simple mean used even in outdoors or at sites.;SOLUTION: A protein modifier and Tb ions are added to a sample liquid to be measured and mixed such that the Tb ions and a protein are coordinated. Then, Ce ions are added and mixed, and the resulting sample solution is excited with near ultraviolet ray and measured for fluorescence of a visible part emitted when the protein is present.;COPYRIGHT: (C)2011,JPO&INPIT
机译:解决的问题:提供一种简便,快速,灵敏的方法,以高重复性和卓越的可操作性对蛋白质进行检测和定量,即使对于生化或化学实验缺乏经验的检测人员也可以轻松进行,并检测痕量蛋白质和解决方案:将蛋白质修饰剂和Tb离子添加到要测量的样品液中并混合,以使Tb离子与蛋白质相协调,从而通过简单的方法对蛋白质进行定量。然后,加入Ce离子并混合,所得样品溶液用近紫外线激发,并测量存在该蛋白质时发出的可见部分的荧光。;版权所有:(C)2011,JPO&INPIT

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