The DNA recombination in eukaryotic cells by recombination system PHIC31 bacteriophage

摘要

(57) [Abstract] The present invention provides a method for obtaining stable integration and specific to eukaryotic cells of nucleic acids. The present invention mediates recombination recombination sites between, but the application of site-specific recombination system using prokaryotic recombinases, such as ΦC31 integrase that is not mediated by hybrid recombination sites between which is formed recombinantly. Therefore, recombination, is irreversible in the absence of additional factors. Also provided are eukaryotic cells that contain a gene encoding a recombinase or recombinase polypeptide.