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Method and system for quantifying the concentration of the fluorescent dye

机译:定量荧光染料浓度的方法和系统

摘要

And quantified using (30) the imaging device the concentration of a plurality of fluorescent dyes contained in (1) in the target sample. The imaging device has a detection wavelength band of the multiple. Providing a reference sample containing multiple units alone given concentration of each of the plurality of fluorescent dye, to obtain the measured intensity at each detection wavelength band of the fluorescence emitted from each sample. The imaged with each detection wavelength band fluorescence image of the target sample by using an imaging device. By performing a calculation using the fluorescence intensity obtained from the target sample and the reference sample to calculate the concentration of each fluorescent dye in the target sample.
机译:并使用(30)成像装置对目标样品中(1)中包含的多种荧光染料的浓度进行定量。成像装置具有倍数的检测波长带。提供参考样品,该参考样品仅在给定多种荧光染料的每种浓度的情况下单独包含多个单元,以获得在从每个样品发出的荧光的每个检测波长带处的测量强度。通过使用成像装置对目标样品的每个检测波长带荧光图像进行成像。通过使用从目标样品和参考样品获得的荧光强度进行计算,以计算目标样品中每种荧光染料的浓度。

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