Amino acid selective labeling of protein synthesis method

摘要

The object of the present invention is that a cell-free protein synthesis system using a wheat germ extract, a method for synthesizing a protein of interest labeled amino acids selectively. In accordance with the present invention, in a cell-free protein synthesis system comprising a wheat germ extract, in the case of labeling with stable isotopes glutamate alanine, or aspartic acid, is metabolized to aspartic acid and glutamic acid alanine, a protein of interest under conditions in which glutamate response that, reactive, aspartic acid is metabolized to glutamic acid does not inhibit protein synthesis and inhibits the reaction that is metabolized to glutamic and aspartic acid and / or to perform a translation reaction of said purpose protein method is provided.