首页> 外国专利> Optical characteristic measuring apparatus using this method and the optical property measurement of the fluorescent sample

Optical characteristic measuring apparatus using this method and the optical property measurement of the fluorescent sample

机译:使用该方法的光学特性测量装置和荧光样品的光学特性测量

摘要

PROBLEM TO BE SOLVED: To accurately determine total spectral radiance factors from which the effects of fluorescence are removed without having to use a fluorescent reference sample or perform complicated calibration work thereof.;SOLUTION: A reflection spectral radiance factor Rx(λ) from which the effects of fluorescence are removed is determined in the following procedure on the basis of a bispectral luminescent radiance factor F(μ, λ) approximate to a sample; the spectral distributions I1(λ) and I2(λ) of illumination lights I1 and I2 having different spectral distributions; and the observed total spectral radiance factors Bx1(λ) and Bx2(λ) of the sample separately illuminated by I1 and I2. 1: Theoretical fluorescent spectral radiance factors F1(λ)=∫F(μ, λ)×I1(μ)dμ/I1(λ) and F2(λ)=∫F(μ, λ)×I2(μ)dμ/I2(λ) are computed on the basis of F(μ, λ) and I1(λ) and I2(λ). 2: Rx(λ) is computed on the basis of the following simultaneous equations that Bx1(λ)=Rx(λ)+F1(λ)×K(λ) and that Bx2(λ)=Rx(λ)+F2(λ)×K(λ) in which Bx1(λ) and Bx2(λ) are the sum of Rx(λ), F1(λ)×K(λ), and F2(λ)×K(λ).;COPYRIGHT: (C)2007,JPO&INPIT
机译:解决的问题:无需使用荧光参考样品或执行复杂的校准工作即可准确确定去除了荧光影响的总光谱辐射因子;解决方案:反射光谱辐射因子Rx(λ)从在以下步骤中,基于近似于样品的双光谱发光辐射因子F(μ,λ)确定荧光的去除效果。具有不同光谱分布的照明光I1和I2的光谱分布I1(λ)和I2(λ);以及分别由I1和I2照射的样品的观察到的总光谱辐射因子Bx1(λ)和Bx2(λ)。 1:理论荧光光谱辐射因子F1(λ)=& F(μ,λ)× I1(μ)dμ/ I1(λ)和F2(λ)=&int F(μ ;,λ× I2(μ)dμ/ I2(λ)是基于F(μ,λ)和I1(λ)和I2(λ)计算的。 2:根据以下联立方程来计算Rx(λ),其中Bx1(λ)= Rx(λ)+ F1(λ)×K(λ),并且Bx2(λ)= Rx(λ)+ F2(λ)× K(λ)其中Bx1(λ)和Bx2(λ)是Rx(&lambda),F1(&lambda)× K( λ)和F2(λ)× K(λ).; COPYRIGHT:(C)2007,JPO&INPIT

著录项

  • 公开/公告号JP4617436B2

    专利类型

  • 公开/公告日2011-01-26

    原文格式PDF

  • 申请/专利号JP20050112337

  • 发明设计人 井村 健二;

    申请日2005-04-08

  • 分类号G01J3/443;G01J3/50;G01N21/64;

  • 国家 JP

  • 入库时间 2022-08-21 18:18:37

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号