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It mixes with the lymphocyte group which is acquired the production mannered null intended film protein of the hybridoma which
It mixes with the lymphocyte group which is acquired the production mannered null intended film protein of the hybridoma which
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机译:它与所获得的淋巴细胞群混合,产生杂交后的原目的膜蛋白。
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摘要
PROBLEM TO BE SOLVED: To provide a method for preparing a hybridoma producing an antibody recognizing the three dimensional structure of a cellular membrane protein.;SOLUTION: This method for preparing a monoclonal antibody specific to a target protein is characterized by comprising steps of mixing a group of lymphocytes obtained by immunity by using the target protein as an antigen with myeloma cells showing the protein on the surface of the cells; selectively approaching the lymphocytes showing a membrane-binding type monoclonal antibody specific to the protein in the lymphocyte group towards the myeloma cells through a specific binding of the membrane-binding type antibody with the membrane protein existing on the surface of the myeloma cells; and fusing both cells.;COPYRIGHT: (C)2006,JPO&NCIPI
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