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External fertilization and culture of human embryo external fertilization of culture solution and human embryo and culture method

机译:人胚的外受精培养法人胚的培养液和受精体外培养法

摘要

Safety is high, external fertilization and culture of the human embryo which, can be able obtain the fertilized egg whose quality is good raise pregnancy ratio the culture solution. As for the culture solution for this culture, although it fertilizes the spermatozoon and the ovum of the person, although the human embryo to 16 cell periods is cultured from the 1st culture solution and after the fertilizing, are used although, with the 2nd culture solution which is used and the human embryo to blastodermic vesicle period is cultured from morula stage the 3rd culture solution which is used it has. The 1st - 3rd culture solution, contains glucose, the lactate and the pyruvic acid salt, at the same time clearly it is bloodless. Glucose density the 1st culture solution is highest, the 2nd culture solution is lowest. Lactate density and pyruvic acid salt density the 3rd culture solution are lowest.
机译:安全性高,可以进行人胚的外部受精和培养,可以得到优质的受精卵提高妊娠率的培养液。作为该培养的培养液,虽然使人的精子和卵子受精,但是从第1培养液到受精后培养16个细胞周期的人胚,但与第2培养液一起使用。所使用的第3种培养液是从桑ula期到人胚至胚盘囊泡期的培养。第一至第三种培养液,包含葡萄糖,乳酸盐和丙酮酸盐,同时显然无血。第一培养液的葡萄糖浓度最高,第二培养液的葡萄糖浓度最低。第三培养液的乳酸浓度和丙酮酸盐浓度最低。

著录项

  • 公开/公告号JPWO2009122541A1

    专利类型

  • 公开/公告日2011-07-28

    原文格式PDF

  • 申请/专利权人 乾 裕昭;

    申请/专利号JP20080534212

  • 申请日2008-03-31

  • 分类号C12N5/02;C12N5/07;

  • 国家 JP

  • 入库时间 2022-08-21 18:17:32

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