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The DNA associated with the genes encoding the α subunit of the second isozyme of anthranilate synthase Rice

机译:与编码邻氨基苯甲酸合酶水稻第二同工酶α亚基的基因相关的DNA

摘要

This invention provides a DNA sequence capable of encoding an enzyme protein which is the alpha -subunit of the first isozyme of rice anthranilate synthase (ASA); and a DNA capable of encoding an enzyme protein which is the alpha -subunit of the second isozyme of ASA. Transformed plants having a high tryptophan content can be regenerated by culturing the plant cells having introduced therein such a recombinant vector which contains, at a position downstream of a promoter, an inserted DNA fragment carrying the above DNA sequence. This invention also provides a DNA having a promoter activity for the rice ASA gene, which DNA can be used as a promoter for introducing a useful exogenous gene into rice or other plants and expressing the gene therein.
机译:本发明提供了一种DNA序列,它能编码一种酶蛋白,该蛋白是水稻邻氨基苯甲酸合酶(ASA)的第一个同工酶的α-亚基。以及能够编码作为ASA第二同工酶的α-亚基的酶蛋白的DNA。色氨酸含量高的转化植物可以通过培养导入了这样的重组载体的植物细胞来再生,该重组载体在启动子的下游位置含有插入的带有上述DNA序列的DNA片段。本发明还提供了对水稻ASA基因具有启动子活性的DNA,该DNA可以用作将有用的外源基因导入水稻或其他植物中并在其中表达该基因的启动子。

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