In the said cationicity high polymer solution which freezes the said anionic high polymer solution which dissolves the culture mannered null anionic high polymer of the animal cell which uses the high-molecular complex

摘要

PPROBLEM TO BE SOLVED: To provide a manufacturing method of a polymer composite, using a plurality of highly reactive polymer compounds, which uniformly contains these polymer compounds and can be formed into an arbitrary shape and easily further into a porous body, the polymer composite obtained by the manufacturing method, and a culturing method of animal cells using the polymer composite. PSOLUTION: One polymer compound A of two kinds of polymer compounds reactive with each other is dissolved in a solvent, and the resultant polymer compound A solution is frozen into an arbitrary shape. The frozen product of the polymer compound A solution is immersed in a polymer compound B solution, obtained by dissolving the other polymer compound B in a solvent compatible with the solvent for the polymer compound A, and is caused to gel. Preferably, the solvent for dissolving the polymer compound B is a mixed solvent of the solvent compatible with the solvent for the polymer compound A and a solvent exhibiting a freezing point-depressing action. The thus-obtained polymer composite can be made into the porous body by vacuum freeze-drying, and the porous body is suitably employed as a carrier for culturing animal cells. PCOPYRIGHT: (C)2006,JPO&NCIPI