Viral antigen detection method and viral antigen detection kit

摘要

[PROBLEMS] To provide a virus antigen detection method and a virus antigen detection kit that can hardly detect a false positive reaction even when a non-human-derived capture antibody or detection antibody is used and that can detect a virus antigen with high accuracy. is there. A method for detecting a viral antigen contained in a human specimen containing an anti-heterologous immunoglobulin antibody, wherein a non-human-derived capture antibody is immobilized on a carrier, and the non-human-derived detection antibody is labeled with a labeling substance. Turn into. Next, adding a blocking antibody having the same epitope as the epitope recognized by the anti-heterologous immunoglobulin antibody, which is common to the capture antibody and the detection antibody, and binding it to the anti-heterologous immunoglobulin antibody And the step of reacting the human specimen with the capture antibody and the detection antibody to bind the capture antibody and the detection antibody to the virus antigen simultaneously or in this order. Subsequently, the labeling substance of the detection antibody bound to the virus antigen is detected. A bridge is not formed between the capture antibody and the detection antibody, and false positives are unlikely to occur.