Enzyme Immunoassay Using Enzyme-Labeled Antibody

摘要

It was able to provide a method for carrying out an enzyme immunoassay such as ELISA or sandwich ELISA, which hardly inhibited by antiseptics and has high reactivity and sensitivity similar or superior to the performance in the case of the use of an antibody labeled using POD derived from horseradish, by carrying out the enzyme immunoassay by labeling a POD derived from Basidiomycetes and using the labeled antibody under an immobilized state. Additionally, even when antiseptics including sodium azide are added, the POD activity is hardly inhibited so that it was able to carry out the enzyme immunoassay with good reactivity and sensitivity.