Detection Device of Screen-Printed Electrode With High Sensitivity

摘要

A detection device for detecting Escherichia coli in a sample includes: an electrode coated with a metal layer, which is further bound with a first nucleic acid sequence; and a second nucleic acid sequence bound with a liposome having an electrochemical material. The second nucleic acid sequence competes with the first nucleic acid sequence for a complementary binding ability, and the liposome is broken to release the electrochemical material. Then, the release electrochemical material is determined so as to estimate whether a third nucleic acid sequence specific for E. coli exists in the sample, where the third nucleic acid sequence is complementary with the first nucleic acid sequence. A trace amount (10⁻¹⁵ mole) of first nucleic acid sequence can be detected using the detection device, which is has advantages of low price, speedy reaction, portability and minimization etc. and can be used for detecting other molecules.