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SCREENING METHOD FOR EFFICIENTLY OBTAINING USEFUL MICROBIAL STRAINS FROM MICROBIAL SAMPLE OBTAINED FROM NATURAL ENVIRONMENT AND REAGENT AND SCREENING KIT TO BE USED THEREFOR
SCREENING METHOD FOR EFFICIENTLY OBTAINING USEFUL MICROBIAL STRAINS FROM MICROBIAL SAMPLE OBTAINED FROM NATURAL ENVIRONMENT AND REAGENT AND SCREENING KIT TO BE USED THEREFOR
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机译:从自然环境和试剂获得的微生物样品中有效获取有用的微生物菌株的筛选方法,以供参考
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摘要
A screening method for efficiently obtaining useful microbial strains from a sample comprises a first step of inoculating a culture medium with the sample potentially containing multiple microbial cells to culture the sample in the presence of a cell enlarger; a second step of introducing an esterase substrate fluorescent stain into the culture medium; a third step of isolating enlarged microbial cells stained in the second step according to a micromanipulation method to produce multiple culture media for establishment each inoculated with a single microbial cell; a fourth step of culturing the microbial cell in each of the culture media for establishment; and a fifth step of selecting culture media for establishment, in which cell proliferation has been confirmed, among the multiple culture media for establishment cultured in the fourth step.
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