DNA Microarray for Quantitative Detection of Microbial Processes in the Oilfield

摘要

A DNA microarray having correctly designed target nucleotide sequences bound thereto generates a quantifiable signal when the microarray hybridizes a field sample nucleotide sequence that indicates a microbial process in a field sample from an oilfield, for instance from downhole. Such DNA microarrays may be designed and manufactured to detect microbial production of hydrogen sulfide, organic acids, surfactants, and gases as well as thermophilic bacterial activity. The field sample nucleotide sequences may be tagged with a fluorescent molecular label, so that the DNA microarrays may generate signals, such as fluorescent signals that may be measured and quantified to provide a more accurate way to correlate bacterial processes in the oilfield than presently available methods.