首页>
外国专利>
Expression Cloning Methods In Filamentous Fungi
Expression Cloning Methods In Filamentous Fungi
展开▼
机译:丝状真菌表达克隆方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
Methods for screening a polynucleotide library for a polypeptide with a property of interest in a filamentous fungal host cell, in a manner which allows quick and easy subsequent characterization of the polypeptide, using an expression cloning vector comprising at least a polynucleotide encoding a selectable marker in which the translation initiation start site of the marker-encoding sequence comprises a crippled consensus Kozak sequence, a fungal replication initiation sequence, and a promoter with a cloning-site into which the library is cloned, and a transcription terminator.
展开▼