Cultured silkworm cells capable of highly efficient baculovirus production and protein production

摘要

Known cell lines derived from silkworm exhibit low propagation efficiency of BmNPV. Accordingly, systems using known culture cell lines derived from silkworms take a long time to establish recombinant viruses, and are not suitable for preparation of virus solutions with high titers. The present invention provides a cell line Bme21 (FERM P-20852) that is derived from a silkworm embryo and is highly susceptible to BmNPV or its variant having the same biological characteristics. The present invention also provides a method of producing a recombinant virus, a method of producing a recombinant protein, a method of increasing efficiency of recombinant virus production, and a method of increasing efficiency of recombinant protein production, using the cell line Bme21 or its variant.