Methods and compositions for the use of a Vaccine against coccidiosis, preparation of multivalent Vaccine, vector, Cell isolation

摘要

Claim 1: a vaccine to protect Poultry coccidiosis Eimeria Infections, characterized in that it comprises a vaccine recombinant Avian adenovirus vector, which comprises a promoter operably linked to a Hydrophobic Signal sequence, which includes a Nucleic Acid encoding a Membrane Anchor domain.A multiple cloning site for insertion of a open reading frame (ORF), with which it is possible to insert an ORF in the same framework with The Hydrophobic Signal sequence, a polyadenylation signal and a Genome of Avian adenovirus.Claim 2: Vaccine against coccidiosis of claim 1, wherein the ORF encodes an Antigen of interest selected from the group consisting of a truncated R56 Antigen of Eimeria maxima, a tfp250 truncated Antigen of Eimeria maxima and 82 kDa Antigen of TRUNC Ado of Eimeria maxima.Claim 5: Vaccine against coccidiosis of claim 1, wherein Said Avian adenovirus Genome is a Genome of Fav 8.Claim 6: Vaccine against coccidiosis of claim 1, wherein Said recombinant Avian adenovirus vector also comprises a Cutting Sequence immediately towards the 5 'end of the cloning site for insertion of the ORF of interest, where the produc To the expression of the Vector is a soluble product.Claim 7: Vaccine against coccidiosis of claim 1, wherein Said Nucleic Acid encodes an Antigen selected from the group consisting of: (a) a truncated R56 Antigen that includes the sequence of nucleotides 70 - 1035 of the complete sequence of R56, Q EU shown in seq. Id.HL: 13, but does not Encode the Protein Sequence complete R56, shown in seq. ID no: 2; (b) an Antigen R56 truncated fragment encoding the truncated R56, consists of 24 amino acids - 345 of seq. ID no: 2, or a 24 amino acid fragment - 345 of seq. Id.HL: 2; (c) a tfp250 truncated Antigen that includes the sequence of 6448 - 7083 nucleotides of the complete sequence of tfp250, shown in seq. Id: 16 NS, but does not Encode the Protein sequence tfp250 thorough, illustrated in seq. ID no: 4; and (d) an antigen tfp250 is truncated in the Nucleic Acid Sequence comprising 6448 - 7083 nucleotides of seq. Id.HL: 16.Claim 24: a recombinant Avian adenovirus vector characterized in that it comprises a promoter operably linked to a Hydrophobic Signal Sequence comprising a Nucleic Acid encoding a Membrane Anchor Domain, a multiple cloning site for insertion of d And an ORF of interestIt is possible to insert an ORF of interest in the same framework with The Hydrophobic Signal sequence, a polyadenylation signal and a Genome of Avian adenovirus. Claim: a 40 isolated Cell which comprises the recombinant Avian adenovirus vector of claim 24.