Methods and Compositions on the basis of Shiga toxin type 2 Protein. Purified polypeptide. Ant stx2 Antibody. Kit.

摘要

Compositions containing polypeptides of stx2 full length does not include the epitope of the monoclonal antibody 11E10. Methods for producing antibody specific for stx2 11E10 Protein epitope of stx2. Methods for treating a Subject that is developing or is at risk of developing, a disease associated with Shiga toxin (e.g., haemolytic uraemic syndrome and diseases associated with infection with E. Coli and S. dysneteriae) with a polypeptide that includes 11E10 epitope Antibody or with an anti stx2 was developed using the methods of the application. Detection of stx2 in a sample using Antibodies that were developed using the methods of the application.Claim 1: a composition for Stimulating an immune response against the stx2 Vehicle comprising an acceptable for pharmaceutical use and at least a Purified polypeptide, the polypeptide comprising: (i) SEC ID no: 1; (ii) SEC ID NSS: 1 and 2; or (iii) SEC ID: 1, 2, NSS and (3); and a scaffold - not stx2 Protein, where the SEC ID no: 1, id: Parts 1 and 2 secs or NSS NSS id: 1, 2,And 3 are inserted into the scaffold of the Protein not stx2 where said polypeptide has the antigenicity of stx2. Claim 2: the composition of claim 1, wherein Said Protein scaffold comprises a substantially identical to the stx1, or a fragment thereof.Claim 7: a Purified polypeptide comprising: (i) SEC ID no: 1; (ii) SEC ID NSS; 1 and 2; or (iii) SEC ID: 1, 2, and 3; and a scaffold Protein not stx2, where the SEC ID no: 2 secs NSS ID 1 and 2, or secs NSS id: 1, 2, and 3 are inserted into the scaffold of the Protein not stx2 And where the said polypeptide has the antigenicity of stx2.Claim 1: The Purified polypeptide of any of claims 1 to 19, where is toxoideado Said polypeptide. Claim 21: an isolated Nucleic Acid Molecule that encodes the polypeptides of any of claims 1 to 19.Claim 23: a Vector capable of Expressing a Vector comprising Said polypeptide, Nucleic Acid Molecule isolated claim 21. Claim 2: a host cell containing the Vector of claim 14.Claim 1: a Pharmaceutical composition for Inducing an immune response in a subject to stx2, Said composition comprising Pharmaceutical compositions of any of claims 1 to 8.Claim: a 31 Antibody anti stx2, or a fragment thereof, that specifically binds to the epitope 11E10 Protein of Type 2 (stx2) of the Shiga toxin polypeptides comprising any of claims 1 - 19, where this Antibody Inhibits the binding of an Antibody 11 E10 (ATCC 1907 11E10), Chimeric Antibody, Humanized Antibody to the Protein or 11E10 stx2.Claim 38: a Pharmaceutical composition comprising any of the Antibodies of Claims 31 to 37 and a vehicle acceptable for pharmaceutical use. Claim: a hybridoma Cell Line producing any of the Antibodies of Claims 31 to 37.