Method of Preparation of Erysipelothrix rhusiopathiae Surface Protective Antigen Mutant in Escherichia coli

摘要

A variant of Erysipelothrix rhusiopathiae surface protective antigen SpaA protein or of a shortened form of SpaA (”SpaA) in which a portion of SpaA protein is deleted for protection from Erysipelothrix rhusiopathiae infection and a process for preparing the same are provided. Introduction of amino acid substitution at a specific site in the amino acid sequence of SpaA or ”SpaA protein provides a variant of SpaA or ”SpaA protein which is immunogenic and is expressed in E. coli as inclusion bodies. The variant of SpaA or ”SpaA protein of the present invention may easily be recovered and purified since it is expressed in E. coli as inclusion bodies.