首页> 外国专利> method of detecting u0446u0438u0442u043eu0433u0435u043du0435u0442u0438u0447u0435u0441u043au043eu0433u043e and u0446u0438u0442u043eu0442u043eu043au0441u0438u0447u0435u0441u043au043eu0433u043e action of environmental factors in the cells of the thyroid gland

method of detecting u0446u0438u0442u043eu0433u0435u043du0435u0442u0438u0447u0435u0441u043au043eu0433u043e and u0446u0438u0442u043eu0442u043eu043au0441u0438u0447u0435u0441u043au043eu0433u043e action of environmental factors in the cells of the thyroid gland

机译：检测 u0446 u0438 u0442 u043e u0433 u0435 u043d u0435 u0442 u0438 u0447 u0435 u0441 u043a u043e u043e u0433 u043e和 u0446 u0438 u0442 u043e u0442 u043e u043a u0441 u0438 u0447 u0435 u0441 u043a u043e u0433 u043e环境因子在甲状腺细胞中的作用

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摘要

the invention aims to simplify and cheaper ways of obtaining cells, improving the reliability, coverage, and, in general,effectiveness evaluation of the different factors in thyroid cells of experimental animals and humans.the technical result is achieved in that the cells for the study conducted by fixation of formaldehyde, the dissociation of cells by 50% contheir method of strokes of dry slides, staining d'azur - u044du043eu0437u0438u043du043eu043c on u0440u043eu043cu0430u043du043eu0432u0441u043au043eu043cu0443.by means of light microscope analyze 1000 u0444u043eu043bu043bu0438u043au0443u043bu044fu0440u043du044bu0445 u0442u0438u0440u043eu0446u0438u0442u043eu0432 (cells), determine the rate of 4 u0446u0438u0442u043eu0433u0435u043du0435u0442u0438u0447u0435u0441u043au043eu0433u043e (cells with u043cu0438u043au0440u043eu044fu0434u0440u0430u043cu0438, u043au043bu0435u0442u043a and the nuclear u043fu0440u043eu0442u0440u0443u0437u0438u044fu043cu0438,cells with u043cu0435u0436u044au044fu0434u0435u0440u043du044bu043cu0438 bridges and the amount of cells with u0446u0438u0442u043eu0433u0435u043du0435u0442u0438u0447u0435u0441u043au0438u043cu0438 violations) and 11 indicators u0446u0438u0442u043eu0442u043eu043au0441u0438u0447u0435u0441u043au043eu0433u043e actions (cells produced form of the kernel, u0434u0432u0443u044fu0434u0435u0440u043d s cells, u0442u0440u0435u0445u044au044fu0434u0435u0440u043du044bu0435 cellscells with stacked cores, the amount of cells with three or more nuclei, mitosis, condensation u0445u0440u043eu043cu0430u0442u0438u043du0430, pyknosis, karyolysis, karyorrhexis, u0430u043fu043eu043fu0442u0438u0447u0435u0441u043au0438u0439 index and vacuoles cytoplasmic held).

机译：本发明旨在简化和廉价获得细胞的方法，提高实验动物和人的甲状腺细胞中不同因子的可靠性，覆盖率以及总体上评价其有效性。通过固定甲醛进行，通过干玻片的笔划以50％的方法分解细胞，对d'azur- u0440 u043e u043c u043c u04c u0437 u0438 u043d u043d u043e u043c染色u043d u043e u0432 u0441 u043a u043e u043c u0443。使用光学显微镜分析1000 u0444 u043e u043b u043b u043b u0438 u043a u0443 u043b u044f u04f u0440 u043d u044b u044b u0445 u0442 u0438 u0440 u043e u0446 u0438 u0442 u043e u0432（单元格），确定4个 u0446 u0438 u0442 u043e u0433 u0435 u043d u0435 u0442 u0438 u0447 u0435 u0441 u043a u043e u0433 u043e（具有 u043c u0438 u043a u043a u0440 u043e u044f u0434 u0440 u0430 u043c u0438， u043a u043b u0435 u0432 u0432 u043f u0440 u043e u04 42 u0440 u0443 u0437 u0438 u044f u043c u0438，具有 u043c u0435 u0436 u044a u044f u0434 u0435 u0440 u043d u044b u043c u043c的单元格和具有的单元格数量u0446 u0438 u0442 u043e u0433 u0435 u043d u0435 u0442 u0438 u0447 u0435 u0441 u043a u0438 u043c u0438违规）和11个指示器 u0446 u0438 u0442 u043e u0442 u043 u043a u0441 u0438 u0447 u0435 u0441 u043a u043e u0433 u043e操作（单元产生的内核形式， u0434 u0432 u0443 u044f u0434 u0435 u0440 u043d s单元， u0442 u0440 u0435 u0445 u044a u044f u0434 u0435 u0440 u043d u044b u0435具有堆叠核心的细胞，具有三个或更多核的细胞数量，有丝分裂，凝结 u0445 u0440 u043e u043c u0430 u0442 u0438 u043d u0430，峰裂，核解，核溢血， u0430 u043f u043e u043f u0442 u0438 u0447 u0435 u0441 u043a u0438 u0439指数和液泡保持在细胞质中。

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公开/公告号EA201000068A1

专利类型
公开/公告日2011-06-30

原文格式PDF
申请/专利权人 УЧРЕЖДЕНИЕ РАМН НАУЧНО-ИССЛЕДОВАТЕЛЬСКИЙ ИНСТИТУТ ЭКОЛОГИИ ЧЕЛОВЕКА И ГИГИЕНЫ ОКРУЖАЮЩЕЙ СРЕДЫ ИМ. А.Н. СЫСИНА РАМН;
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申请/专利号EA20100000068
发明设计人 ЛЮДМИЛА ПЕТРОВНА;ЮРИЙ АНАТОЛЬЕВИЧ;НАТАЛИЯ НИКОЛАЕВНА;АЛЕКСАНДРА АНДРЕЕВНА;
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申请日2009-11-18
分类号G01N33/48;G01N1/28;
国家 EA
入库时间 2022-08-21 18:06:43

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7. 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