INVITRO IMMOBILIZED BACILLUS MEGATERIUM CELL FOR THE PRODUCTION OF DEMETHYLATED COLCHICOSIDES FROM COLCHICINES AND PROCESS THEREOF

摘要

The present invention provides a process of microbial transformation of colchicines in to 3-demethylated colchicodine by immobilized bacterial cells, Bacillus megaterium through a bioreactor. The use of this microorganism in immobilized form has made possible to scale up the process of production of 3-demethylated colchicoside with high microbial transformation process, high efficiency and economic feasibility. The process of the present invention provides the 3-O-glycosyl derivatives in high yields and purity.