SCAR AND A KIT FOR THE AUTHENTICATION OF SINGLE DRUG TUKHM-E-KASOOS (CUSCUTA REFLEXA) AND ITS ADULTCRANT AFTIMOON VILAYATI (CUSCUTA CHINENSIS)

摘要

"SCAR and a kit for the Authentication of Single Drug Tukhm-e-Kasoos (cuscuta reflexa) and its Adulterant AFTIMOON VILAYATI (Cuscuta chinensis)". This invention relates to an oligonucleotide primer set (SCAR primers) for authentification of genuine single Unani drug Tukhm-e-kasoos (Cuscuta reflexa) (la) and its adulterant, Aftimoon Vilayti (Cuscuta chinensis) (lb) comprisinga forward and a reverse primer5"CGGATTCCTGGAGTTTATGC-3", 5"GTGAATGCGAGGACGAGTTG3"and5" CGTGATTTCGGACGTGAAAA3" , 5" CGTGACGGTGTACTCGAATG 3", respectively and a kit for authentication of Cuscuta species, which comprises, i) an oligonucleotide primer set for the genuine drug Cuscuta reflexa that comprises a forward primer and a reverse primer were selected, ii) from the group consisting of, (a) an oligonucleotide primer set that comprises a forward oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO:3 (5"CGGATTCCTGGAGTTTATGC-3") and a reverse oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO:4 (5"-GTGAATGCGAGGACGAGTTG-3", (b) an oligonucleotide primer set for the adulterant Cuscuta chinensis that comprises a forward oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO:5(5"-CGTGATTTCGGACGTGAAAA-3") and a reverse oligonucleotide primer having the nucleotide sequence set forth in SEQ ID NO:6 (5"-CGTGACGGTGTACTCGAATG-3"),.