Method for producing a Protein domain is a Protein that is a functional Protein; process to selectively Express exons encoding this Protein; recombinant Gene integrated into the eukaryotic Cell Gene, which comprises the exon; DNA constructs to produce such prot

摘要

The present invention provides a method of producing functional protein domains, which are fragments of primary translational products consisting of one or more distinct protein domains encoded by specific subsets of exonic sequences. The method is based on the integration, by a single homologous recombination event, of a regulatory unit into the eukaryotic gene coding for the primary translational product at the level of the intronic genomic region immediately adjacent to the exonic sequences which code for the functional protein domain.