PRIMERS AND PROBES FOR DETECTING GENITAL HPV GENOTYPES

摘要

Oligonucleotides (ON) that can be used as primers for amplification of nucleic acid regions of genital human papilloma virus (HPV), are new. Oligonucleotides (ON) that can be used as primers for amplification of nucleic acid regions of genital human papilloma virus (HPV) are new. Specifically they have sequence comprising 25 nucleotides (SEQ ID No.:1), especially for use as forward primer, and sequence comprising 23 nucleotides (SEQ ID No.:7), especially for use as reverse primer 5'-CARGC(I or N)AAAWWWKTDAARGAYTGT (SEQ ID No.:1) 5'-ARYGGYTSYARCCAAAARTGRCT (SEQ ID No.:7) where R = A or G; W = T or A; K = T or G; I = inosine; N = A,T,G or C; D = A, T or G; Y = C or T and S = C or G. Independent claims are also included for: (1) oligonucleotide (ON1) useful as probe for detection and/or determination of genital subtypes of HPV having any of 24 sequences reproduced; (2) specified mutant of ON or ON1; (3) oligonucleotides (ON2) having a sequence that is complementary to ON or ON1 over its entire length; (4) primer pair for amplification of a nucleic acid region of a genital HPV comprising at least one ON or ON1, or their specified mutants; (5) nucleic acid (NA1) comprising at least one region of ON1 (or its mutants or complements) plus one or more additional regions of at least one nucleotide (nt); (6) nucleic acid (NA2) that is complementary to NA1 over its entire length; (7) amplifying a region of genital HPV, present in a biological sample, that uses at least one each of forward and reverse primers as defined; (8) detecting/determining a genital HPV genotype by hybridization with ON1, its mutant or complement; (9) array for detecting/determining the genotype of HPV that contains the new oligonucleotides and/or nucleic acids that contain them; and (10) kit for detecting/determining the genotype of a genital HPV containing any of the new primers, primer pairs or probes.