PROCESS FOR PREPARING THE IMMUNOSORBENT TRENBOLONE-3-CARBOXYMETHYLOXIME-OVALBUMIN-CARBOXYMETHYL-CELLULOSE

摘要

The invention relates to a process for preparing the immunosorbent Trebolone-3-Carboxymethyloxime-Ovalbumin-Carboxymethyl-Cellulose (Tren-3-CMO-OVA-CM-Cellulose) used in affinity chromatography for the purification of anti-trebolone specific antibodies. According to the invention, the process consists in dissolving CM-cellulose, N-hydroxy-succinimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide in dimethylformamide, followed by ovalbumin admixture in carbonate buffer, centrifugation, removal of supernatant, washing the resulting precipitate, CM-Cellulose-Ovalbumin, with distilled water, and renewed suspension thereof in sodium carbonate buffer, followed by the coupling reaction of trebolone-3-CMO with the conjugate CM-Cellulose-Ovalbumin, to obtain the desired immunosorbent, namely Trebolone-3-CMO-Ovalbumin-CM-Cellulose, which is washed with a solution of ethyl alcohol in distilled water and stored at a temperature of 4°C.