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Set of primers, probes, method and kit for genetic polymorphism S447X genotipadodel the lpl gene.

机译:遗传多态性S447X genotipadodel lpl基因的引物,探针,方法和试剂盒的集合。

摘要

Set of primers, probes, method and kit for genotyping of genetic polymorphism S447X the LPL gene.; The present invention relates to a set of primers, probe, method and kit for genotyping of genetic polymorphisms, more specifically for genotyping of genetic polymorphism S447X the LPL gene by PCR amplification of a DNA sample using the primer set claimed, and fluorescence detection and genotyping of genetic polymorphism using the probe assembly claimed. The main advantages of the present invention are rapidly that enables large-scale genotyping as the PCR reaction and detection of the fluorescent signal are simultaneous, allocation automated obtainable genotypes immediately after the reaction, high sensitivity allowing samples genotyped using very low concentrations of DNA, and less risk of contamination being a homogeneous assay.
机译:LPL基因多态性S447X基因分型的引物,探针,方法和试剂盒。本发明涉及用于遗传多态性的基因分型的引物,探针,方法和试剂盒的集合,更具体地,涉及通过使用要求保护的引物组的DNA样品的PCR扩增,通过PCR扩增LPL基因的遗传多态性S447X LPL基因的基因分型,以及荧光检测和基因分型。使用要求保护的探针组件的遗传多态性。本发明的主要优点是迅速进行大规模基因分型,因为同时进行PCR反应和荧光信号检测,反应后立即分配自动可获得的基因型,高灵敏度允许使用非常低的DNA浓度对样品进行基因分型,并且均质测定的污染风险较小。

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