Method and system for measuring the fluorescence lifetime in the frequency domain with high levels of background signal.

摘要

Method of measuring the fluorescence lifetime in the frequency domain with high levels of background signal.; Measuring the average lifetime of fluorescent emission in the frequency domain it is hampered by the presence of background signals. a method is proposed for detecting said lifetime even when the fluorescence is much lower than the bottom, without requiring a calibration sample, based on the measurement corresponding to different modulation frequencies of the excitation signal. By doing this, the background signal remains constant, while the signal interest rate changes. The signal of interest can be recovered by performing a cancellation of the common signal. This technique can be used to eliminate the effect of light scattered excitation and autofluorescence.