Method of detecting local events intracellular calcium release.

摘要

Method of detecting local events intracellular calcium release.; The present invention relates to a method of detecting local events intracellular calcium release comprising the steps of:; - acquiring a sequence of two-dimensional images of a particular section of one or more cells, including information relating to activity of intracellular calcium;; - normalizing said images;; - a statistical analysis of the spatiotemporal distribution of pixels in said sequence of normalized images to determine a region of interest, and determine that the regions of interest are candidates to contain a local event of intracellular calcium release interest; Y.; - performing the following steps automatically and prior to that statistical analysis way:; - detect global events calcium release in one or more of the images of said succession of images; Y; - perform such statistical analysis only on the images which have not been detected global calcium release events.