Methods for rapid forensic analysis of mitochondrial DNA and mitochondrial DNA heteroplasmia caraterización.

摘要

A forensic method comprising the steps of: (i) determining a first molecular mass of a first amplification product of a primer amplicon identifier mitochondrial DNA by mass spectrometry; and (ii) comparing the first molecular mass to a second molecular mass of a second amplicon identifier mitochondrial DNA, where the primer and the second identifiers amplicons mtDNA contain the same conserved sequences for hybridization of the same pair of primers, where method does not comprise nucleic acid sequencing of the first amplification product, and wherein the second molecular mass of the second mitochondrial DNA amplicon identifier is indexed to a subject and contained in a database.