PRIMERS AND PROBES TO DETECT GENOTYPES OF THE GENITAL HUMAN PAPILOMA VIRUS.

摘要

Ewq nucleotide array (array) for the detection and / or determination of the genotype of a human papillomavirus existing in a biological sample, consisting of a solid support with a surface and at least one first oligonucleotide or bound nucleic acid molecule to said support surface, said oligonucleotide or nucleic acid molecule is suitable as a probe for the analysis of the HPV E1 gene or a part thereof, for the detection and determination of a genital human HPV genotype, chosen from among the group consisting of: a) HPV genotype-specific oligonucleotides having the nucleotide sequences defined in SEQ ID NO 19, 32, 41, 44, 48, 82 and 117 to 135, b) oligonucleotides, which have a nucleotide sequence mutated with respect to one of the oligonucleotides of part a), namely a deletion or addition of 1 to 10 nucleotides or a substitution of 1 to 3 nucleotides in one of the nucleus sequences Ótides described in section a), c) oligonucleotides, which have a nucleotide sequence, which throughout its length is complementary to the nucleotide sequence of an oligonucleotide according to section a) or b), d) nucleic acid molecules that consist at least one region, which has one of the nucleotide sequences defined in sections a) to c) and one or more additional regions of a total length of at least one nucleotide and e) mixtures of the oligonucleotides according to sections of a) ac) and / or nucleic acid molecules according to section d).