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Gene technology processors to determine genes memory.
Gene technology processors to determine genes memory.
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机译:基因技术处理器确定基因记忆。
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摘要
A method identifying a candidate gene or genes involved in transcription dependent memory of associative learning comprising the steps of: (a) training non-human animals to induce the formation of transcription dependent memory in these animals; (B) extracting RNA from brain tissue of said trained in step (a) animals; (C) synthesizing DNA probes using the RNA extracted in step (b); (D) hybridizing the DNA probes synthesized in step (c) to complementary DNA sequences on processors microarrays containing DNA sequences from genes of the genome of said animals, wherein a signal is produced upon hybridization of said probes to complementary DNA sequences; (E) detecting the signal produced in step (d); and (f) performing a statistical comparison between the detected in step signal (e) and the signal detected in a control for each gene using a transformation algorithm signal signal, wherein said control is obtained according to a process comprising the steps of: (i) extracting RNA from brain tissue of non-human animals trained to induce formation controlling independent memory but not transcription-dependent memory formation transcription associated with said control animals; (Ii) synthesizing DNA probes using the RNA extracted in step (f) (i); and (iii) hybridizing the DNA probes synthesized in step (f) (ii) to complementary DNA sequences on processors provisions containing DNA sequences from genes of the genome of control animals, wherein a signal is produced upon hybridization of said probes to complementary DNA sequences; and wherein memory formation in the animal of step (a) is induced using a spaced training protocol and memory formation in the control animals is induced using a massed training protocol; and both the spaced training protocol and the massed training protocol use Pavlovian conditioning procedures with a conditioned stimulus and an unconditioned stimulus that are temporally paired; and wherein a statistically significant difference between the detected in step (e) and the signal detected in a control for each gene indicates a signal candidate memory gene involved in associative memory dependent transcription.
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