The invention relates to a process for the industrial production of a vaccine against Japanese encephalitis, characterized in that it comprises the following steps: a) culturing cells from a cell line, b) inoculation of the culture of cells obtained by Japanese encephalitis virus in the presence of a viral multiplication medium, c) propagation and multiplication of the virus on the cells, d) harvesting of the viral multiplication medium constituting a suspension of virus produced by the cells, e) purification of the viral suspension by at least one ion exchange chromatography step and a gel permeation step, f) formulation and pharmaceutical form of the viral suspension to ensure its preservation until its use. The invention also relates to a vaccine against Japanese encephalitis characterized in that it comprises Japanese encephalitis virus obtained by culturing on cells from a cell line, and in that the cellular DNA content is less than 100 μg / dose.
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