METHODS AND COMPOSITIONS FOR THE PREPARATION AND USE OF FIXED-TREATED CELL-LINES AND TISSUE IN FLUORESCENCE IN SITU HYBRIDIZATION

摘要

This invention relates to methods for the detection of one or more mRNAtranscripts in paraffin-embedded tissue by "mRNA liberation in fixed-treatedtissue or 'MLIFTT"'. This method includes treating the tissue with ammonia-ethanol and sodium borohydride combined with pressure cooking of the tissue.The chemical treatments reduce the tissue autofluorescence and the physicaltreatments overcome the interference created by the fixation-induced chemicalbonds. The methods of the present invention can be utilized to identify aplurality of mRNA transcripts in a microarray format.