首页> 外国专利> METHOD, PARTICULARLY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA), FOR IN VITRO DETECTION OF AMYLOID BETA AUTOANTIBODIES, MICROTITER PLATE, AND TEST KIT

METHOD, PARTICULARLY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA), FOR IN VITRO DETECTION OF AMYLOID BETA AUTOANTIBODIES, MICROTITER PLATE, AND TEST KIT

机译:淀粉样β自体抗体,微量滴定板和试剂盒的体外检测方法,尤其是酶联免疫吸附测定(ELISA)

摘要

A method, particularly an enzyme-linked immunosorbent assay (ELISA), for the in-vitro detection of Aβ autoantibodies in human serum and/or plasma contains the following steps: preparing an antigen-coated solid phase; incubating the solid phase with a blocking solution; incubating the solid phase with a sample to be examined; immunological detection of the Aβ autoantibodies on the solid phase; and reading the detected results of the solid phase using a reading tool. According to the invention, the preparation of the antigen-coated solid phase advantageously includes incubating the solid phase with a coating solution in which the antigen is dissolved, said antigen having a peptide sequence selected from the group SEQ ID No. 1, SEQ ID No. 2 or SEQ ID No. 3.
机译:一种用于体外检测人血清和/或血浆中Aβ自身抗体的方法,特别是酶联免疫吸附试验(ELISA),其包括以下步骤:制备抗原包被的固相;用封闭液孵育固相;将固相与要检查的样品一起孵育;固相中Aβ自身抗体的免疫学检测;然后使用读取工具读取检测到的固相结果。根据本发明,抗原包被的固相的制备有利地包括将固相与其中溶解有抗原的包被溶液一起温育,所述抗原具有选自SEQ ID No.1,SEQ ID No的肽序列。 2或SEQ ID No.3。

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