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The method, in particular enzymes - linked immunosorbent assay (elisa), for the in vitro detection of amyloid beta autoantibodies, microtiter plate and a test kit

机译:该方法,特别是酶联免疫吸附测定法(elisa),用于体外检测淀粉样β自身抗体,微量滴定板和检测试剂盒

摘要

In the case of a process, in particular an enzyme - linked immunosorbent assay (elisa), for the in vitro detection of aβ - autoantibodies in human serum and / or plasma, comprising the steps of providing an antigen - coated solid phase, incubating the solid phase with a blocking - solution, incubating the solid phase with a sample to be examined, immunological detection of the aβ - auto-antibodies to the solid phase and reading out of the detection result on the solid phase with the aid of a reading device, it is advantageous if the provision of the antigen - coated solid phase comprising incubating the solid phase with a coating - solution comprises, in which an antigen is released, which is a peptide - sequence selected from the group consisting of seq id no. 1, seq id no. 2 or seq id no. 3.
机译:在用于体外检测人血清和/或血浆中的α-自身抗体的方法,特别是酶联免疫吸附测定(elisa)的情况下,包括提供抗原包被的固相的步骤,带有封闭溶液的固相,将固相与待检样品一起孵育,对固相免疫检测aβ-自身抗体,并借助读取装置从固相中读出检测结果有利的是,提供包括将固相与包被溶液一起孵育的抗原包被的固相的方法包括,其中释放出抗原的肽(选自肽seq id no。 1,序列号2或序号3。

著录项

  • 公开/公告号DE102009034119A1

    专利类型

  • 公开/公告日2011-01-27

    原文格式PDF

  • 申请/专利权人

    申请/专利号DE20091034119

  • 发明设计人

    申请日2009-07-20

  • 分类号G01N33/564;G01N33/543;

  • 国家 DE

  • 入库时间 2022-08-21 17:47:46

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