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CELL GROWTH, INDUCTION AND LYSIS IN AN ANTIBODY-COATED MICROPLATE FOR USE IN AN ELISA
CELL GROWTH, INDUCTION AND LYSIS IN AN ANTIBODY-COATED MICROPLATE FOR USE IN AN ELISA
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机译:ELISA法用于抗体包被的微孔板的细胞生长,诱导和裂解
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摘要
A competitive assay to determine the presence and concentration of an intracellular analyte (e.g., cAMP) in a sample is provided. All of the steps of the assay can be performed on the same assay plate, thereby eliminating the need to transfer the cells from a tissue culture plate on which the cells are grown, induced and lysed to a separate assay plate. The assay procedure includes combining, in a reaction chamber provided with a capture antibody, an antibody for the analyte, the sample to be assayed, and a conjugate of the analyte and an enzyme such as alkaline phosphatase. The mixture is incubated and washed and an enzyme labile substrate (e.g., a chemiluminescent, fluorescent or colorimetric substrate) is added. The assay can also be performed with a tagged analyte (e.g., an analyte having a radioactive or fluorescent tag) instead of an enzyme conjugate.
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