首页> 外国专利> METHOD FOR PRODUCING TARGET PROTEINS BY DELETING OR AMPLIFYING ibpA AND/OR ibpB GENE CODING FOR INCLUSION BODY-ASSOCIATED PROTEINS

METHOD FOR PRODUCING TARGET PROTEINS BY DELETING OR AMPLIFYING ibpA AND/OR ibpB GENE CODING FOR INCLUSION BODY-ASSOCIATED PROTEINS

机译：通过删除或扩增包含体结合蛋白的ibpA和/或ibpB基因编码生产靶蛋白的方法

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摘要

The present invention relates to a method for producing target proteins by deleting or amplifying ibpA and/or ibpB genes coding for inclusion body-associated proteins. The present invention provides two methods for producing target proteins using ibpA and/or ibpB genes coding for inclusion body-associated proteins of E. coli, whose effect on the production of target proteins have not yet been reported. The first method of the present invention is to enhance the secretory production and activity of target proteins using ibpA and/or ibpB genes-deleted bacteria. The second method of the present invention is to enhance the production of target proteins in the cytoplasm and also convert the target proteins from soluble form to insoluble inclusion body, using ibpA and/or ibpB gene-amplified bacteria.

机译：本发明涉及通过缺失或扩增编码包涵体相关蛋白的ibpA和/或ibpB基因来生产靶蛋白的方法。本发明提供了两种使用编码大肠杆菌的包涵体相关蛋白的ibpA和/或ibpB基因生产靶蛋白的方法，尚未报道其对靶蛋白产生的影响。本发明的第一种方法是使用ibpA和/或ibpB基因缺失的细菌来增强靶蛋白的分泌产生和活性。本发明的第二种方法是使用ibpA和/或ibpB基因扩增的细菌来增强细胞质中靶蛋白的产生，并且还将靶蛋白从可溶性形式转化为不溶性包涵体。

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公开/公告号EP1604015B1

专利类型
公开/公告日2011-09-21

原文格式PDF
申请/专利权人 KOREA ADVANCED INST SCI & TECH;
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申请/专利号EP20030816262
发明设计人 LEE SANG YUP;PARK SI JAE;HAN MEE JUNG;
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申请日2003-07-10
分类号C12N1/21;
国家 EP
入库时间 2022-08-21 17:59:27

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专利
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Vascular320Effect of gastrin-releasing peptide (GRP) on vascular inflammationSignal transduction - Heart323A new synthetic peptide regulates hypertrophy in vitro through means of the inhibition of nfkb324Inducible fibroblast-specific knockout of p38 alpha map kinase is cardioprotective in a mouse model of isoproterenol-induced cardiac hypertrophy325Regulation of beta-adrenoceptor-evoked inotropic responses by inhibitory G protein, adenylyl cyclase isoforms 5 and 6 and phosphodiesterases326Binding to RGS3 and stimulation of M2 muscarinic acetylcholine receptors modulates the substrate specificity of p190RhoGAP in cardiac myocytes327Cardiac regulation of post-translational modifications, parylation and deacetylation in LMNA dilated cardiomyopathy mouse model328Beta-adrenergic regulation of the b56delta/pp2a holoenzyme in cardiac myocytes through b56delta phosphorylation at serine 573Nitric oxide and reactive oxygen species - Vascular331Oxidative stress-induced miR-200c disrupts the regulatory loop among SIRT1, FOXO1 and eNOS332Antioxidant therapy prevents oxidative stress-induced endothelial dysfunction and Enhances Wound Healing333Morphological and biochemical characterization of red blood cell in coronary artery diseaseCytoskeleton and mechanotransduction - 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V Garcia-Martinez, C Lopez Sanchez, W Hamed, 2016

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