Fluid dispenser, fluid dispensing method and assay apparatus for assay in utilizing attenuated total reflection

摘要

A multiple pipette assembly (19, 26, 126) is used for the purpose of sample immobilization for surface plasmon resonance (SPR) assay, and dispenses and introduces sample fluid (21, 27) of a sample to a sensing surface (13a) of a sensor unit (12, 120). The sensor unit includes a flow channel (16, 122) having first and second orifices (16a, 16b), and the sensing surface, positioned in the flow channel, for detecting the reaction of the sample. First and second pipette devices (19a, 19b, 26a, 26b, 126a-126c) access the orifices. First and second pumps (52, 147) are connected with respectively the pipette devices, for pressurization to dispense the sample fluid through the pipette devices , or for decompression to aspirate the sample fluid through the pipette devices. The pumps are driven in a manner independent from one another. While the first and second pipette devices move away from the sensor unit, the pumps are caused to decompress a space in the first and second pipette devices and the flow channel.