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RHODANOBACTER GINSENOSIDIMUTANS KCTC22231T-DERIVED GINSENOSIDE GLYCOSIDASE, AND USE THEREOF

机译:根瘤菌人参皂甙KCTC22231T衍生的人参皂甙糖苷酶及其用途

摘要

The present invention relates to Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase protein and to the use thereof, wherein the protein has an activity of selectively hydrolyzing a specific site bond of ginsenoside to transform protopanaxadiol (PPD) saponins into a deglycosylated highly active material which is absorbable in vivo. In detail, the present invention relates to an amino acid sequence constituting the protein, to a nucleic acid sequence for encoding the protein, to a recombinant vector comprising the nucleic acid sequence, to a transformant transformed by the vector, to a method for preparing Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase by transformant cultivation, to a method for transforming PPD-type major saponins into a rare saponin type that is absorbable in vivo using the protein, and to a composition for transforming PPD-type saponins containing the protein as an active ingredient into a saponin type that is easily absorbable in vivo.
机译:本发明涉及人参Rhodanobacter ginsenosidimutans KCTC22231T衍生的人参皂苷糖苷酶蛋白及其用途,其中该蛋白具有选择性水解人参皂苷的特定位点键的活性,以将原人参二醇(PPD)皂苷转化为可吸收的去糖基化的高活性物质。体内。详细地,本发明涉及构成该蛋白质的氨基酸序列,用于编码该蛋白质的核酸序列,包含该核酸序列的重组载体,由该载体转化的转化体,罗丹杆菌的制备方法。人参糖苷酶KCTC22231T衍生的人参皂苷糖苷酶,通过转化体培养,将PPD型主要皂苷转化为稀有皂苷类型的方法,该蛋白可在体内被该蛋白吸收,还涉及用于转化PPD-的组合物含有该蛋白质作为活性成分的皂苷型皂苷型成为在体内容易吸收的皂苷型皂苷。

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