ICG DOPED SILICA NANOPARTICLES FOR BIOLOGICAL IMAGING AND PREPARATION METHOD THEREOF

摘要

The present invention relates to indocyanine green (ICG)-doped silica nanoparticles, which can be used as a near-infrared fluorescent nanoprobe for biological imaging, and a preparation method thereof. More specifically, the invention relates to silica nanoparticles doped with ICG conjugated with a positively charged molecule such as polyethylenimine (PEI), aminopropyl trimethoxysilane or bovine serum albumin, and a preparation method thereof comprising the steps of: conjugating ICG with a positively charged molecule as a core; and incorporating the positively charged molecule-conjugated into silica nanoparticles. The ICG-doped silica nanoparticles synthesized according to the preparation method of the invention has advantages in that the size thereof is easily controlled at the nano level and in that they can be surface-modified via versatile routes and have low leakage, low toxicity, low photo bleaching, high sensitivity enough to apply for in vitro and in vivo imaging, and sustained stability.