METHOD OF ISOLATION OF STEM CELL POPULATIONS FROM PERIPHERAL BLOOD USING SIZED-BASED SEPARATION (ELUTRIATION)

摘要

Disclosed is a method for isolating multiple populations of stem cells in at least one step from a peripheral blood sample without using positive selection, therefore enabling a "no-touch" method to isolate different stem cell populations without contaminating the stem cell populations with positive selection agents (e.g., antibodies, etc ) In particular, the present disclosure relates to flow-rate separation of stem cell populations using elutriation (sized-based separation) to negatively exclude cells based on size Different fractions or subfractions comprise an increase in yield of Very Small Embryonic-like Stem cells (VSELs), MSCs or HSCs as compared to non-fractionated apheresis product.