Device for determining concentration of spectrally distinguishable species in a biological sample

摘要

A fluorometry device for determining concentration of spectrally distinguishable species in a biological sample, the device comprising: a light source adapted to provide a source beam;a plurality of sample wells; a plurality of optical devices comprising an excitation filter adapted to filter the source beam, an emission filter adapted to filter fluorescent emission light from the samples, and a beamsplitter to separate the source beam from the fluorescent emission light, wherein each of the plurality of optical devices is coupled to a movable platform, wherein the excitation filter, the emission filter, and the beamsplitter provide a first narrow wavelength range for excitation and a second narrow wavelength range for emission; a detector adapted to receive the fluorescent emission light emitted from the samples; and an optical element positioned to collimate the emission light and to reduce non-uniformities in the emission light prior to the emission light impinging the beamsplitter and/or positioned to collimate the excitation light and to reduce non-uniformities in the excitation light prior to the excitation light impinging the beamsplitter.